Molar Ratio of Insert to Vector

Generally ligations are tested at 1:3, 1:1, and 3:1 insert:vector molar ratios.

 kb (insert length)
 ng (vector amount)
 kb (vector length)

You need ng of insert for a 1:1 molar ratio
kb(insert length)   x  ng of vector  =  ng of insert needed for a 1:1 ratio

kb(vector length)

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