Linear DNA: µg → pmol of DNA Ends

Parameter
 µg (DNA)
 kb (DNA)


Result
 pmol DNA ends
µg DNA  x  pmol  x  106pg  x  1  x  2  x  kb  =  pmol DNA ends




660pg 1µg N 1000bp

N is the number of nucleotides (in kb),
660pg/pmol is the average molecular weight of a single nucleotide pair,
2 is the number of ends in a linear DNA molecule, and
kb/1000bp is a conversion factor for kilobases to base pairs

      

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