Oligo Calculator [ Usage: ]
Input Oligo sequence in the box.
Click Clear-button for Next sequence.
Avilable Cut & Past from another application
SEQUENCE  

COMPLEMENT

Length  Melting Temperature (Tm) ℃ 
%GC content  Molecular Weight: daltons (g/M) 
1 OD is same as  nmole.µg
Information on the Tm value:
oligos <18 bases calculates
Tm = 4℃: x (number of G's + C's in the primer) + 2℃: x (number of A's + T's in the primer) .  
 
oligos ≧18 bases calculates .  
Tm =((ΔH * 1000 / (ΔS + 41.3)) - 273.15 - 21.5971) (CONC. 0.05M-salt 250pmol-primer)
      

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