|ssRNA's Data of preservation test
|This is the PCR comparison data using ssRNA preserved in RNA SHIELDER at 4 ℃. For preservation test of RNA SHIELDER, we prepared 2 kinds of BCR/ABL and TERT prepared by in vitro transcription and being purified by RNA purification kit. We prepared samples of ssRNA solution containing RNA SHIELDER (+) and RNA SHIELDER (-) using RNA SHIELDER and DEPC water so that concentration of ssRNA should become 8X105 copy/µL and, then we kept them in refrigerator at 4 ℃. We collected constant amount from stored samples kept at 4 ℃ at the determination point of each time course and performed reverse transcription in 20 µL system. Then, we performed PCR with reverse transcription product using genes primers and hydrolysis probe and traced time course of Cp values. Followings are results of preservation test for TERT genes.
< Genes determined：TERT 4℃ storage >
|It is observed that ssRNA kept in RNA SHIELDER ( - ) having only DEPC water decomposes gradually and Cp values are increasing over time. On the other hand, in ssRNA solution containing RNA SHIELDER (+), Cp values do not almost fluctuate from Cp value of -80 ℃ control and it is understood that decomposition of ssRNA is being suppressed. ), Cp values do not almost fluctuate from Cp value of -80 ℃ control and it is understood that decomposition of ssRNA is being suppressed.