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About Sending Samples
  • Please undergo purification without fail to get rids of PCR products, for desalination and elimination of extra primers and dNTP.
  • We recommend plasmid DNA prepared by commercial plasmid purification kit.
    (We will not recommend simple purification method such as boil method due to possibility of degradation of quality of analysis data.)
  • Please confirm in advance by electrophoresis and so on that there is no impurity such as residual RNA and Genome DNA.
  • We accept purification of PCR products as option service.
  • Please use PCR grade water for adjustment of volume.
  • Please fill in 0.5ml of sample into Eppendorf tube and write "Tube No." of "Application Form of Contracted Service" on the cap of the tube.
  • In case you are asking us to undergo synthesis of primers, please declare so in the application form.
Please send sample in room temperature.
Mailing sample volume
Basic α Course
Sampleper 1 reaction3µg
Primerper 1 reaction6.4pmol/µL
· Please prepare needed volume of samples taking into consideration of volume per reaction.
· Please prepare needed unlabeled primers in case you are going to send primers.
· Please consult us if it's difficult to adjust preparation of above samples.
Advance Course
Samplelength of less than 1kbpmore than 4µg/1sample;
length of less than 2kbpmore than 8µg/1sample
length of less than 3kbpmore than 12µg/1sample
Primermore than 10µL of 6.4pmol
· Please prepare needed volume of samples and unlabeled primers taking into consideration of volume per reaction.
· Please consult us if other than the above case or if it's difficult to adjust sample preparation.
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